Effect of Different Dietary Selenium Sources on Growth Performance, Antioxidant Capacity, Gut Microbiota, and Molecular Responses in Pacific White Shrimp Litopenaeus vannamei

This study investigated the effect of different dietary selenium (Se) sources on growth performance, antioxidant capacity, gut microbiota, and molecular responses Pacific white shrimp Litopenaeus vannamei. Four Se (sodium selenite, L-selenomethionine, yeast, or nanoparticles) were added to purified diets 0.4 mg Se/kg diet for ( 1.60 ± 0.14 g ). Each treatment was randomly assigned 3 replicated tanks 30 in each tank (500 L). After 8 weeks breeding, L-selenomethionine yeast significantly increased weight gain compared with sodium selenite treatment, while decreased hepatosomatic index other groups. The superoxide dismutase glutathione peroxidase activities hepatopancreas fed catalase activity malondialdehyde content composition β-diversity microbiota markedly changed group. abundances Rubrobacter Rubritalea, Winogradskyella Motilimonas, Photobacterium specially altered by nanoparticles, respectively. group showed lower complexity interspecies interactions. RNA-seq analysis that “arachidonic acid metabolism”-related genes enriched groups; “peroxisome” “drug metabolism–other enzymes”-related nanoparticle Vibrio, associated amino lipid metabolism. Pseudoalteromonas, Silicimonas, Roseovarius, Halomonas inhibited expression genes. These results suggested organic Se, especially selenomethionine, is an effective feed supplement promote maintain health utilization fatty a diet.